direct staining method

Objective To compare peripheral blood mononuclear cells(PBMC) staining and fresh whole blood staining methods for detecting antigen-specific cytotoxic T lymphocytes(CTL) with MHC pentamers in chronic hepatitis B patients.

目的分别采用分离外周血单个核细胞(PBMC)法和全血直接染色法的组织相容性抗原-肽五聚体(MHCpentamers)流式细胞技术定量分析慢性乙型肝炎(下称慢性乙肝)患者体内乙型肝炎病毒(HBV)特异性的细胞毒性T淋巴细胞(CTL),并对两种方法进行比较。

AODC was applied to researching the total number of marine bacterioplankton at 2005 in Bohai Bay.

主要对几种细菌荧光显微计数法的原理和应用做了介绍,并于2005年利用吖啶橙染色直接计数法对渤海湾海域浮游细菌总数进行了的调查研究。